1 µl random primers (250 ng/µl) (Thermo Fisher)

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Thermo Fisher 1 µl random primers (250 ng/µl)
1 µl Random Primers (250 Ng/µl), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/250+ng+random+primers/pmc09700454-111-10-48
Average 90 stars, based on 1 article reviews

1 µl random primers (250 ng/µl) - by Bioz Stars, 2026-10

90/100 stars

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Article Title: GS2 as a retinol transacylase and as a catalytic dyad independent regulator of retinylester accretion.
Article Snippet: GS2 (PNPLA4; iPLAg) is the smallest member of the patatin-like family of phospholipases (PNPLA).. It was initially identified by its ability to hydrolyze retinylesters (RE) in cell homogenates, and was later found to esterify retinol using a variety of acyl donors.. In the present study we set out to determine its cellular function and examined its impact on RE status in 293T cells transfected with GS2, GS2-M1 (a non-translatable mutant of GS2) and empty vector, in fibroblasts isolated from normal and GS2-null donors and in SCC12b and in a somatic cell knock-out of GS2 (SCC12b-GS2 ), that we generated by homologous recombination.

Article Title: Induction of regulatory T cells by a murine β-defensin.
Article Snippet: Total RNA was isolated with TRIzol Reagent (Invitrogen, Karlsruhe, Germany) from mouse skin samples. .. Then 1 mg total RNA was converted by reverse transcription into cDNA, using 250 ng random primers and 200 U SuperScript II (both Invitrogen). .. Quantitative real-time PCR was performed using a fluorescence temperature cycler (LightCycler; Roche, Mannheim, Germany) and corresponding sets of specific primers.

Article Title: Variations in the expression of vasotocin and isotocin receptor genes in the gilthead sea bream Sparus aurata during different osmotic challenges.
Article Snippet: Genomic DNA (gDNA) was eliminated using the on-column RNase-free DNase digestion (included in the kit) at 25 C for 15 min. RNA quality was checked in a Bioanalyzer 2100 with the RNA 6000 Nano kit (Agilent Technologies), whereas RNA quantity was measured spectrophotometrically at 260 nm in a BioPhotometer Plus (Eppendorf). .. Brain total RNA (2 lg) was reverse-transcribed in a 20-lL reaction using 250 ng random primers (InvitrogenTM, LifeTechnologies) and 200 U Superscript III reverse transcriptase (InvitrogenTM, LifeTechnologies) with the manufacturer’s first strand buffer (1 final concentration), DTT (5 mM final concentration), and dNTPs (0.5 mM final concentration) at 25 C for 5 min, 50 C for 60 min, and 70 C for 15 min. PCR amplifications were carried out with 1 U Platinum Taq DNA Polymerase (InvitrogenTM, Life Technologies) with the first strand cDNA (corresponding to 10 ng of input total RNA), manufacturer’s PCR buffer (1 final concentration), 200 nM of each sense and antisense primers, 200 lM dNTP mixture, and 1.5 mM MgCl2 in a total volume of 20 lL. ..

Article Title: TiO 2 nanoparticles synergize with substrate mechanics to improve dental pulp stem cells proliferation and differentiation.
Article Snippet: Multiple studies exist on the influence of TiO2 nanoparticle uptake on cell behavior.. Yet little is known about the lingering influence of nanoparticles accumulation within the external environment which is particularly important to stem cell differentiation.. Herein, dental pulp stem cells were cultured on hard and soft polybutadiene substrates, where 0.1 mg/mL rutile TiO2 nanoparticles were introduced once, 24 h after plating.

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Article Title: Suppression of Extravillous Trophoblast Vascular Endothelial Growth Factor Expression and Uterine Spiral Artery Invasion by Estrogen during Early Baboon Pregnancy
Article Snippet: .. RT of total RNA from LCM isolates was performed at 42 C for 60 min in a 20-μl reaction volume containing 1 m m each of deoxy (d)-ATP, dCTP, dGTP, and -thymidine triphosphate, 200 U Moloney murine leukemia virus RT, 1× RT buffer, 250 ng random primers (preceding reagents from Invitrogen, Carlsbad, CA), and 40 U RNAguard (Amersham Pharmacia Biotech, Piscataway, NJ). .. The RT reaction was terminated by heat inactivation of the enzyme at 70 C for 15 min and cooled to 4 C. VEGF and sFLT1 mRNA levels were quantified by efficiency-corrected calibrator-normalized relative RT-PCR using LightCycler SYBR Green I technology (Roche Diagnostics).

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Article Title: Effects of di(2-etilhexil) phthalate on human umbilical artery.
Article Snippet: Accepted Manuscript Effects of di(2-etilhexil) phthalate on human umbilical artery R. Azevedo, N. Oliveira, C. Maia, I. Verde PII: S0045-6535(19)30782-9 DOI: https://doi.org/10.1016/j.chemosphere.2019.04.128 Reference: CHEM 23636 To appear in: ECSN Received Date: 30 November 2018 Revised Date: 15 March 2019 Accepted Date: 16 April 2019 Please cite this article as: Azevedo, R., Oliveira, N., Maia, C., Verde, I., Effects of di(2-etilhexil) phthalate on human umbilical artery, Chemosphere (2019), doi: https://doi.org/10.1016/ j.chemosphere.2019.04.128.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

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